Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit -40304ES

YeasenSKU: 40304ES50

Size: 50 T
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Description


Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit is an Annexin Fluor 647 labeled with Annexin V as a probe to detect the occurrence of early apoptosis, which can be detected by flow cytometry or other fluorescence detection equipment.

The detection principle is that in normal living cells, phosphotidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS reverses from the inner side to the surface of the cell membrane and is exposed to the extracellular environment. Annexin V is a Ca2+ -dependent phospholipid binding protein with a molecular weight of 35-36 kD that binds PS with high affinity. Phosphatidylserine can bind to the membrane of early-apoptotic cells by exposing it laterally.

In addition, Propidium Iodide (PI) is provided in this kit to distinguish between surviving early cells and necrotic or late apoptotic cells. PI is a kind of nucleic acid dye, which can not penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells and make the cell nucleus red. Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-). The apoptotic and necrotic cells were double positive by Alexa Fluor 647 and PI binding staining (Annexin V+/PI+).This kit requires flow cytometry.

Features

  • The reagents in this kit are provided in liquid form for easy use.
  • This kit is commonly used for flow cytometry
  • Under the fluorescence microscope, the color distinction is obvious.

Applications

  • This kit can be used for flow cytometry.
  • This kit can detect the early apoptosis of cells.
  • This kit can distinguish between late apoptotic and necrotic cells.

Specifications

Application The verified application of the kit was to detect apoptotic cells by flow cytometry.
Packing form Bottle

Components

Component Name 40304ES20 (20T) 40304ES50 (50T) 40304ES60 (100T)
40304-A Annexin V-Alexa Fluor647 100 μL 250 μL 500 μL
40304-B PI Staining Solution (20 μg/mL) 200 μL 500 μL 1.0 mL
40304-C 1×Binding Buffer 10 mL 25 mL 50 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 1 year.

Citations & References:

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[5] Han L, Wu Y, Liu F, Zhang H. eIF4A1 Inhibitor Suppresses Hyperactive mTOR-Associated Tumors by Inducing Necroptosis and G2/M Arrest. Int J Mol Sci. 2022;23(13):6932. Published 2022 Jun 22. doi:10.3390/ijms23136932(IF:5.924)

[6] Gao F, Wang Q, Zhang C, et al. RNA methyltransferase METTL3 induces intrinsic resistance to gefitinib by combining with MET to regulate PI3K/AKT pathway in lung adenocarcinoma. J Cell Mol Med. 2021;25(5):2418-2425. doi:10.1111/jcmm.16114(IF:5.310)

[7] Ren L, Hu L, Zhang Y, et al. Cataract-Causing S93R Mutant Destabilized Structural Conformation of βB1 Crystallin Linking With Aggregates Formation and Cellular Viability. Front Mol Biosci. 2022;9:844719. Published 2022 Mar 14. doi:10.3389/fmolb.2022.844719(IF:5.246)

[8] Ding M, Weng C, Fan S, Cao Q, Lu Z. Purkinje Cell Degeneration and Motor Coordination Deficits in a New Mouse Model of Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay. Front Mol Neurosci. 2017;10:121. Published 2017 May 1. doi:10.3389/fnmol.2017.00121(IF:5.076)

[9] Cai C, Dang W, Liu S, et al. Anthrax toxin receptor 1/tumor endothelial marker 8 promotes gastric cancer progression through activation of the PI3K/AKT/mTOR signaling pathway. Cancer Sci. 2020;111(4):1132-1145. doi:10.1111/cas.14326(IF:4.966)

[10] Li X, Yao Q, Huang J, et al. Morin Hydrate Inhibits TREM-1/TLR4-Mediated Inflammatory Response in Macrophages and Protects Against Carbon Tetrachloride-Induced Acute Liver Injury in Mice. Front Pharmacol. 2019;10:1089. Published 2019 Sep 20. doi:10.3389/fphar.2019.01089(IF:3.845)

[11] Jiang Y, Du M, Wu M, et al. Phosphatidic Acid Improves Reprogramming to Pluripotency by Reducing Apoptosis. Stem Cells Dev. 2016;25(1):43-54. doi:10.1089/scd.2015.0159(IF:3.727)

[12] Zhang Q, Wu C, Fan Y, et al. Nucleic acid-targeted pathogen reduction technique in red blood cells by UV-generated oxygen radicals for optimising recipient safety. Transfus Med. 2020;30(1):51-60. doi:10.1111/tme.12654(IF:1.900)

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