BCA (Bicinchoninic acid) method is a widely used method for protein concentration determination at present. Based on the biuret reaction, that is, in an alkaline environment, the protein reduces Cu2+ to Cu+, producing a purple blue complex, which has a high absorbance value at 562 nm. The amount of the reaction product is proportional to the protein concentration. BCA protein concentration determination method is simple, sensitive, fast and stable. The protein standard provided in the kit provides convenience for users to make standard curves.
The BCA protein concentration determination kit can be used for colorimetric assay and microplate assay. The former requires a large amount (100 μL) of protein sample, The ratio of protein sample to BCA working solution is 1:20 (v/v), which reduces the impact of interfering substances. The latter is simple and convenient to operate, requiring only a small amount (10-25 μL) of protein sample. However, because the ratio of protein sample to BCA working solution is 1:8 (v/v), the tolerance concentration of interfering substances is limited to some extent and the minimum detection level is reduced. Our company provides three sizes of BCA protein concentration detection kits, the colorimetric method can be used for 50 times, 250 times and 500 times respectively. The enzyme labeling method can be used for 500 times, 2500 times and 5000 times respectively.
- High sensitivity, with a minimum detection protein of 0.2 μg. The lower limit of detection concentration reaches 10 μg/mL.
- It is faster and takes less time to develop color than the general BCA protein concentration determination kit. It is about 4 times faster than the traditional Lowry method.
- Wide linear range, there is a good linear range in the concentration range of 20-2000 μg/mL.
- It is not affected by the chemicals in most of the samples.
- Protein Quantitation
|Product Type||Protein Quantitation Assay|
|For Use With (Application)||Solution-based Detection, Absorbance|
|For Use With (Equipment)||Spectrophotometer, Microplate Reader|
|20201-A||BCA Reagent A||100 mL||500 mL||2×500 mL|
|20201-B||BCA Reagent B||3 mL||15 mL||2×15 mL|
|20201-C||Protein standard (BSA)||5×1 mL
Reagent A and Reagent B in the kit should be stored at room temperature or 2℃~ 8℃. Protein standard (BSA) can be stored at -25℃ ~ -15℃ for 12 months if it is not used for a long time.
Figure 1. Good linear range, the R2 values of standard curves among different batches were all above 0.995.