FuniCut™ Bsa I GMP-grade -10661ES

YeasenSKU: 10661ES76

Size: 500 U
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Sale price$105.00

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Description

This product is a type IIS restriction endonuclease derived from the recombinant protein encoded by the BsaI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GGTCTCN1/N5-3'. Use to digest plasmids to prepare poly(A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends.

This product is produced in accordance with GMP process requirements and provided in a liquid form.


Specifications

Expression Host

Recombinant E. coli with BasI gene

Reaction Temperature

37℃

Storage Buffer

10 mM Tris-HCl, 0.2 M NaCl, 0.1 mM EDTA, 1mM DTT, 50% Glycerol

Unit Definition

1 unit: The amount of enzyme required to digest 1 μg of substrate DNA within 1 h at 37℃ in a 50 μL system.

Application

1.Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);

2.Digestion of DNA to obtain specific sticky ends;

3.Linearize plasmid template before in-vitro transcription.


Components

Components No.


Name

10661ES03

(1 KU)

10661ES10

(10 KU)

10661ES60

(100 KU)

10661

BsaI GMP-grade (20 U/μL)

50 μL

500 μL

5 mL


Storage

This product should be stored at -25 ~ -15℃ for two years.


Instructions

Experimental methods

50 μL reaction system

This step is suitable for linearization of 1 μg DNA (≥100 nt) and can be scaled up according to experimental needs.

  1. 1.Add the following components in sequence:

Components

Volume

Plasmid DNA

1-2 μg

10×Digestion Buffer 4

5.0 μL

BsaI (20 U/μL)

1.0 μL

RNase-free ddH2O

Up to 50 μL

【Note】10× Digestion Buffer 4(Cat#10668ES): 500 mM Potassium Acetate,200 mM Tris-acetate,100 mM Magnesium Acetate,1 mg/ml OsrHSA, pH7.9@25℃

  1. 2.Incubate at37°C 1 h;
  2. 3.DNA linearization is complete, and subsequent experiments can be performed.


Notes

  1. Heat inactivation condition: incubate at80°C for20min.
  2. Please operate with lab coats and disposable gloves,for your safety.

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