Hieff™ Bst Plus DNA Polymerase (40 U/μL) -14402ES

Save $200.00
YeasenSKU: 14402ES97

Size: 40000 U
Price:
Sale price$235.00 Regular price$435.00

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Description

Hieff™ Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5'-3' exonuclease activity. The enzyme has stronger 5'-3' DNA polymerase activity, strand displacement activity, and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc.

Features

  • Thermophilic DNA polymerase with strong strand displacement activity

Applications

  • Isothermal DNA amplification

Specifications

Polymerase Bst DNA Polymerase
Heat Inactivation Incubation at 85°C for 5 min.
Solution Components 10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃.
Activity Definition 1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C.

Components

Components No. Name 14402ES92 (8,000 U) 14402ES97 (40,000 U)
14402-A Hieff™ Bst Plus DNA Polymerase (40 U/μL) 200 μL 1 mL
14402-B 10 ×Hieff™ Bst Plus DNA Polymerase Buffer 1 mL 3×1 mL
14402-C 100 mmol/L MgSO4 1 mL 3×1 mL

Figures

  • High Sensitivity

Figure 1: Sensitivity Test

Red: 50 copies/T, response less than 15 minutes
Figure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%.

 

  • Stability

Figure 2.Stability testing at the same temperature

Red: 37℃ 0 day  Blue: 37℃ 1 day  Green: 37℃ 3 days  Purple: 37℃ 5 days
0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.

 

Figure 3. -20℃ repeated freeze-thaw stability test

Red: Repeat freeze-thaw 0 times
Blue: Repeat freeze-thaw 5 times
Green: Repeat freeze-thaw 10 times

 

  • Specificity

Figure 4. 48-well NTC-specific assay

48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better.

 

Storage

The product should be stored at -25℃ ~ -15℃ for 2 years. Please avoid repeated freeze-thaw.

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