Transforming growth factor-beta 1 (TGF-β1) is a major pluripotential cytokine with a pronounced immunosuppressive effect. TGF-β1 is one of three closely related mammalian members of the large TGF-β superfamily that share a characteristic cystine knot structure. Mature human TGF-β1 shares 100% aa identity with pig, dog and cow TGF-β1, and 99% aa identity with mouse, rat and horse TGF-β1. TGF-β1 is a key regulator of diverse biological processes in many tissues and cell types, its can be tumorsuppressive through the activation of the Smadmediated signaling pathway. TGF-β1 inhibits the growth factor and oncogene induction of transin/stromelysin, a secreted matrix-degrading metalloprotease.
|Synonyms||CEDLAP, DPD1, latency-associated peptide, TGF beta, TGF beta1, TGFB, TGFB1|
|Source||CHO-derived Human TGF-β1, Ala279-Ser390.|
|Molecular Weight||Approximately 12 kDa under reducing conditions.|
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY
IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ
|Physical Appearance||Sterile Filtered White lyophilized (freeze-dried) powder.|
|Purity||>97% by SDS-PAGE analyses.|
|Biological Activity||Measured by its ability to inhibit the IL-4-dependent proliferation of HT2 mouse T cells. The ED50 for this effect is 0.04-0.2 ng/mL. The specific activity of Recombinant Human TGF-β1 is approximately 2.5 × 104 U/μg, which is calibrated against human TGF-β1 Standard.|
|Endotoxin||< 0.1 EU per 1μg of the protein by the LAL method.|
|Formulation||Lyophilized from a 0.2 μm filtered concentrated solution in 35% Acetonitrile and 0.1% TFA.|
|Reconstitution||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriately buffered solutions.|
Shipping and Storage
The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
1.Avoid repeated freeze-thaw cycles.
2.For your safety and health, please wear lab coats and disposable gloves for operation.
3.For research use only!