UCF.ME™ Salt Active UltraNuclease GMP-grade (250 U/μL) -20159ES

Save $360.00
YeasenSKU: 20159ES25

Size: 25 KU
Price:
Sale price$235.00 Regular price$595.00

Shipping calculated at checkout

Stock:
Only 5 units left
Bulk Quote Inquiry

Description

Product description

Salt Active UltraNuclease is a nonspecific endonuclease and has optimum activity at high salt concentration. Salt can play an important role in minimizing aggregation of protein or virus, which would be helpful for dissociation of DNA & RNA from proteins or other cellular components. In high salt conditions, enzymes are more likely to access the released DNA & RNA and degrade them. Nuclease which has high activity at high salt would be more effective to improve purification processes. The Salt Active UltraNuclease can be used to reduce the viscosity of cell supernatant and cell lysate, increase purification efficiency in high salt condition.

The enzyme can also reduce host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing. 

This product is produced by GMP process requirements and provided in a liquid form with the following features.

  • High purity (≥ 98%)
  • Animal-free production
  • Low Endotoxin
  • High activity at high salt conditions
  • Supported by ELISA Kit

Components

Components No.

Name

20159ES25

(25 KU)

20159ES60

(100 KU)

20159ES80

(1 MU)

20159ES90

(5 MU)

20159

Salt Active UltraNuclease GMP-grade (250 U/μL)

100 μL

400 μL

4 mL

20 mL

Specification

Expression Host

Recombinant, expressed in Escherichia coli

Molecular Weight

24.7kDa

Isoelectric point

9.61

Purity

≥ 99%

Storage Buffer

25 mM Tris-HCl,5 mM MgCl2,500 mM NaCl,50% glycerol

Unit Definition

The definition of one activity unit (U) is the amount of enzyme that causes a △A260=1.0 in   30 minutes at 37℃ in the excess of substrate at the certain condition.

 

Recommended operating conditions

 Condition

Optimal

Effective

Mg2+/Mn2+

10~50mM

1~100mM

pH

8

6.5 ~10

Temperature

37℃

4~42℃

Na+/K+

500mM

300~1200mM

Recommended Usage

Condition

Suggested usage

Protein

100U/mL

Cell lysate

500U/mL

Cell extract

1000U/mL

Viscosity reduction

50U/mL


AAV Production Case

Supplier

Condition (100U/mL, 37℃, 1h)

HCD residualsng/ml)

M*

400mM NaCl

1.42

M*

400mM NaCl,1% Tween

0.68

Yeasen 20159ES

400mM NaCl

0.87

Yeasen 20159ES

400mM NaCl,1% Tween

0.07

Shipping and Storage

The product should be stored at -25 ~ -15℃ for two years. If the product is opened and has been stored at 4℃ for more than one week, we recommend filtering the product to prevent microbial contamination.

Documents:

Manuals

20159_Manual_HB230808_EN.pdf

 

You may also like

Recently viewed