Uracil DNA Glycosylase (UDG/UNG), 1 U/μL -14455ES

YeasenSKU: 14455ES60

Size: 100 U
Price:
Sale price$55.00

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Description

UDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP.

Features

10 U of this product was detected by E.coli 16S rDNA-specific TaqMan qPCR, and the result showed that E.coli genome residue was fewer than 10 copies
No nucleic acid endonuclease, exonuclease and RNase residues
Uracil is the sole base recognized by this enzyme

Applications

Remove ssDNA and dsDNA, but they are inactive to RNA
Eliminate false positive results due to contamination of PCR amplification products.
Compatible with PCR, qPCR, RT-qPCR, etc

Specifications

Expression Host Recombinant E. coli with uracil DNA glycosylase gene
Molecular Weight 24.8 kDa
Purity ≥ 95%(SDS-PAGE)
Heat Inactivation 95°C, 5~10 min
Unit Definition One unit (U) is defined as the amount of enzyme that required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C.

Components

Components No. Name 14455ES60 (100 U) 14455ES76 (500 U) 14455ES96 (10,000 U)
14455 Uracil DNA Glycosylase (UDG/UNG), 1 U/μL 100 μL 500 μL 10 mL

Storage

The product should be stored at -25℃ ~ -15℃ for two years.

Figures

Figure 1.UDG enzyme (0.025 U) fully digested 360 ng of 200 bp dU -DNA

Electrophoresis results of 0.05 U, 0.025 U, 0.0125 U, 0.00625 U, 0.003125 U anti- contamination UDG enzyme with 360 ng of 200 bp dU -DNA incubated at 25°C for 30 min.

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