Hieff NGS™ Fast-Pace DNA Cyclization Kit for MGI is a single-strand cyclization kit specifically designed for MGI platforms. The use of high-quality enzymes and optimized Buffer significantly reaction efficiency, enabling the entire cyclization and digestion process to be completed in less than 30 minutes. This kit is suitable for all standard single-barcode libraries for MGI platforms and is not limited to different MGI sequencing platforms except for the limitations of the library-prep reagents.
- Wide range of use: compatible with mainstream library preparation kits (KAPA, etc.)
- Fast cyclization: a 1-hour rapid cyclization
- High cyclization efficiency: the cfDNA cyclization efficiency>30%,other sample types>40%
- To prepare a single-stranded circular DNA library dedicated to the MGI high-throughput sequencer
|Product Type||NGS Library Preparation|
|Product Line||DNA library Preparation|
|Input DNA||The recommended Input DNA is 1 pmol, if the PCR product is insufficient, down to 0.5 pmol|
|For Use With (Application)||Single Barcode DNA Cyclization Kit for MGI|
|For Use With (Equipment)||MGI Platforms|
|Components No.||Name||13341ES16 (16T)||13341ES96 (96T)|
|13341-A||Splint Oligo||96 μL||576 μL|
|13341-B||Splint Buffer||240 μL||2×720 μL|
|13341-C||Ligase||80 μL||480 μL|
|13341-D||Digestion Buffer||128 μL||768 μL|
|13341-E||Digestion Enzyme||32 μL||192 μL|
-25℃ ~ -15℃ storage, valid for one year.
Figure 1. Comparison of cyclization efficiency of PCR-free library preparation for different fragment lengths.
Method: The PCR-free libraries were prepared with Cat#12202 PCR-free library prep kit.
Results: The library cyclization efficiency of the different library fragments Cat#13341 was higher than that of Supplier M*