Description
Hifair™ IV Reverse Transcriptase is an updated version of Hifair™ Ⅱ Reverse Transcriptase obtained through genetic engineering technology. It has higher cDNA synthesis ability and speed, thermal stability and reaction temperature limit (up to 60°C) than Hifair™ Ⅱ Reverse Transcriptase. The synthesized cDNA product is up to 19.8 kb. Hifair™ IV Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structure or low copy genes.
Specifications
|
Cat# |
11112ES92/11112ES93/11112ES08 |
|
Size |
10,000 U/50,000 U/ 5 mL |
|
Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using Oligo(dT) as primers. |
Components
|
Components No. |
Name |
11112ES92 |
11112ES93 |
11112ES08 |
|
11112-A |
5×HifairTM IV Buffer |
250 μL |
1,250 μL |
25 mL |
|
11112-B |
HifairTM IV Reverse Transcriptase (200 U/μL) |
50 μL |
250 μL |
5 mL |
Storage
The product should be stored at -25℃ ~ -15℃ for 18 months.
Nots
- Keep the experimental area clean; Wear clean gloves and masks during operation; all supplies used in the experiment must be RNase free to prevent RNase.
- All operations should be carried out on ice to prevent RNA degradation.
- High quality RNA samples are recommended for efficient reverse transcription.
- For your safety and health, please wear lab coats and disposable gloves for operation！
- This product is for research use ONLY!