RNase H (5 U/μL) -12906ES

SKU: 12906ES76

Size: 500U
Sale price$95.00

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RNase H (ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA on hybrid DNA/RNA strands, but will not hydrolyze phosphates in single- and double-stranded DNA or RNA diester bonds.

Product Components



(500 U)


(5000 U)


RNase H

100 μL

1 mL


10×RNase H Reaction Buffer*

1 mL

10×1 mL

[Note]:*10×RNase H Reaction Buffer:750 mM KCl,500 mM Tris-HCl,30 mM MgCl2,100 mM Dithiothreitol,pH 8.3 @ 25℃.

Shipping and Storage

All the components are shipped with dry ice and can be stored at -20°C for two years.


1. Remove mRNA before cDNA double-strand synthesis;

2. In RT-PCR/RT-qPCR experiments, remove RNA after the synthesis of one strand of cDNA;

3. Remove poly(A) after Oligo(dT) hybridizes with mRNA;

4. Cleavage of RNA-specific sites.

Definition of enzymatic activity

In 50 μL reaction system, the amount of enzyme required to hydrolyze the RNA-DNA hybrid strand to generate 1 nmol of ribonucleotides was defined as 1 U at 37°C for 20 min.


1) For your safety and health, please wear a lab coat and disposable gloves for operation.

2) This product is only for scientific research purposes!

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