Description
This is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.
Note: G* is the first base of the RNA transcript.
Feature
- dsRNA level lower to about 1/100000
- Comptiable with Trilink CleanCap AG
- High yields comparable to WT
Please find information on the development of this enzyme.
Components
| Components No. | Name | 10628ES10 | 10628ES60 | 10628ES72 | 10628ES86 | 10628ES99 |
| (10 KU) | (100 KU) | (250 KU) | (2,500 KU) | (100 MU) | ||
| 10628 | T7 RNA Polymerase (low dsRNA) (250 U/μL) | 40 μL | 400 μL | 1 mL | 10 mL | 400 mL |
Specifications
|
Source |
Recombinant E. coli with T7 RNA Polymerase gene |
|
Optimum Temperature |
37℃ |
|
Storage Buffer |
50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v/v) glycerin，pH7.9 at 25℃ |
|
Unit Definition |
The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit. |
Related Products
Tris NTPs synergistically decreasing dsRNA
Figures
Shipping and Storage
The products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.