T7 RNA Polymerase (low dsRNA, 250 U/μL) -10628ES

YeasenSKU: 10628ES10

Size: 10 KU
Price:
Sale price$180.00

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Description

This is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.

Note: G* is the first base of the RNA transcript.

Feature

  • dsRNA level lower to about 1/100000
  • Comptiable with Trilink CleanCap AG
  • High yields comparable to WT 

Please find information on the development of this enzyme.

Components

Components No. Name 10628ES10 10628ES60 10628ES72 10628ES86 10628ES99
(10 KU) (100 KU) (250 KU) (2,500 KU) (100 MU)
10628 T7 RNA Polymerase (low dsRNA) (250 U/μL) 40 μL 400 μL 1 mL 10 mL 400 mL


Specifications

Source

Recombinant E. coli with T7 RNA Polymerase gene

Optimum Temperature

37℃

Storage Buffer

50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100,50% (v/v) glycerin,pH7.9 at 25℃

Unit Definition

The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.

 

 

Related Products

Tris NTPs synergistically decreasing dsRNA


Shipping and Storage

The products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

Documents

MSDS

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